The quality of rice has always been an important issue in China, and the effect of rice amylose content on its quality is very important. Determination of amylose can be measured with a starch analyzer, and the current widely used Juliano ratio The color method is used by China's national standard method. Scan WeChat for more:
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Amylose determination of the sample determination of the sample dispersion: Weigh the sample equivalent to 0.10008 crude starch in 100mL volumetric flask by adding 1mL of anhydrous ethanol, fully wet the sample, then add 9mLlmol / L of NaOH solution, placed in a boiling water bath dispersion lOmin Rapid cooling with water to volume. Degreasing: Take 20mL of the dispersion in SOmL stoppered test tube by adding 7mL of petroleum ether, shake off lOmin, let stand for 15min, layered and sucked with the suction pipe connected to the pump, suck the upper layer of petroleum ether . Repeat the operation 2}3 times (2 times for polished rice, 3 times for corn and millet). Determination: After degreasing the alkaline dispersion S.OOmL in a 100mL volumetric flask, add water SOmL, then add 1mLlmol/L acetic acid and 1mL iodine reagent, water to volume. After lOmin was developed, the absorbance was read at 635nm.
In the measurement of amylose, the absorption curve reflects the light absorption ability of the substance at different wavelengths, and it can select the measurement wavelength for quantitative analysis. Usually, the maximum absorption wavelength is selected as the measurement wavelength, and the absorbance is measured at this wavelength, the sensitivity is the highest. It is an absorption curve of starch-iodine test solution issued by the Rice Research Institute of Chinese Academy of Agricultural Sciences. It reflects the ability of the starch solution to absorb light at different wavelengths. 